rabbit anti calbindin (Cell Signaling Technology Inc)
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Rabbit Anti Calbindin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+anti+calbindin/pmc13000480-346-49-53
Average 86 stars, based on 1 article reviews
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Blocking Assay:Article Title: Antibody-assisted selective isolation of Purkinje cell nuclei from mouse cerebellar tissue. Article Snippet: .. Blocking was performed with 5%BSA in PBS at room temperature for 1 h. Next, the slices were stained overnight at 4 Cwith primary antibodies: Article Title: Antibody-assisted selective isolation of Purkinje cell nuclei from mouse cerebellar tissue Article Snippet: .. Blocking was performed with 5% BSA in PBS at room temperature for 1 h. Next, the slices were stained overnight at 4°C with primary antibodies: Article Title: Defective Astrocyte Maturation Drives Cerebellar Neuroinflammation and Degeneration Article Snippet: Subsequently, brains were embedded in optimal cutting medium (Tissue- Tek, VWR), and 50 μm frozen sections were prepared on probe plus microscopy slides (Fisher Sci). .. For permeabilization, a solution of 1% Triton X- 100in PBS was applied for 1 h, followed by blocking in PBS containing 0.5% Triton X- 100 and fish skin gelatin (Electron Microscopy) for 1 h. Primary antibodies Chicken Anti- GFAP (Aves Lab., GFAP, 1:500), Rabbit Anti- IBA1 (FUJIFILM, 016- 20001, 1:200), Rabbit Anti- β- Catenin (Millipore, ABE208, 1:200), Rabbit Anti- NeuN (Cell Signaling Tech., D4G40, 1:200), Staining:Article Title: Antibody-assisted selective isolation of Purkinje cell nuclei from mouse cerebellar tissue. Article Snippet: .. Blocking was performed with 5%BSA in PBS at room temperature for 1 h. Next, the slices were stained overnight at 4 Cwith primary antibodies: Article Title: Antibody-assisted selective isolation of Purkinje cell nuclei from mouse cerebellar tissue Article Snippet: .. Blocking was performed with 5% BSA in PBS at room temperature for 1 h. Next, the slices were stained overnight at 4°C with primary antibodies: Article Title: Cerebellar microglia-derived IL-17A mitigates autism-related behavioral and synaptic deficits. Article Snippet: .. The slices were stained overnight at 4 °C with primary antibodies, including rabbit anti-Iba1 (1:500, Wako, Cat# 016-20001), mouse anti-IL17A (1:100, eBioscience, Cat# 14-7179-82), goat anti-IL17RA (1:200, R&D, Cat# AF448), Incubation:Article Title: Neonatal hyperoxia exposure causes cerebellar lesions and behavioral abnormalities in rats. Article Snippet: .. Subsequently, the 528 sections were incubated overnight at 4°C with monoclonal rat anti-BrdU 529 (1:1,000 in PBS, Bio-Rad Laboratories, Hercules, CA), rat anti-MBP (1:200 in 530 PBS, Merck Millipore, Burlington, MA), or Article Title: Defective Astrocyte Maturation Drives Cerebellar Neuroinflammation and Degeneration Article Snippet: Subsequently, brains were embedded in optimal cutting medium (Tissue- Tek, VWR), and 50 μm frozen sections were prepared on probe plus microscopy slides (Fisher Sci). .. For permeabilization, a solution of 1% Triton X- 100in PBS was applied for 1 h, followed by blocking in PBS containing 0.5% Triton X- 100 and fish skin gelatin (Electron Microscopy) for 1 h. Primary antibodies Chicken Anti- GFAP (Aves Lab., GFAP, 1:500), Rabbit Anti- IBA1 (FUJIFILM, 016- 20001, 1:200), Rabbit Anti- β- Catenin (Millipore, ABE208, 1:200), Rabbit Anti- NeuN (Cell Signaling Tech., D4G40, 1:200), Article Title: Reduced SK channel control of mesolimbic dopamine neuron firing drives reward seeking adaptations in chronic pain Article Snippet: .. Slices were then incubated with Article Title: Neonatal hyperoxia exposure causes cerebellar lesions and behavioral abnormalities in rats Article Snippet: .. Subsequently, the sections were incubated overnight at 4 °C with monoclonal rat anti-BrdU (1:1,000 in PBS, Bio-Rad Laboratories, Hercules, CA), rat anti-MBP (1:200 in PBS, Merck Millipore, Burlington, MA), or Binding Assay:Article Title: Dual mechanisms of supporting cell regeneration in the neonatal mouse cochlea Article Snippet: Tissues were washed another three times with PBS at 10-minute intervals and mounted with ProLong Gold Antifade Mountant (Invitrogen) then coverslipped. .. The following primary antibodies were used: goat anti-Sox2 (1:200; R&D Systems), rabbit anti-fatty acid binding protein 7 (FABP7, 1:200, Abcam), mouse anti-Connexin 26 (1:400; Thermo Fisher), rabbit anti-EAAT1/GLAST (1:500; Abcam), rat anti-ki67 (1:100; Thermo Fisher), rabbit anti-cellular retinoic acid binding protein 1 (CRABP1, 1:200, VWR-Proteintech), goat anti-Galectin-1 (1:50, R&D), Electron Microscopy:Article Title: Defective Astrocyte Maturation Drives Cerebellar Neuroinflammation and Degeneration Article Snippet: Subsequently, brains were embedded in optimal cutting medium (Tissue- Tek, VWR), and 50 μm frozen sections were prepared on probe plus microscopy slides (Fisher Sci). .. For permeabilization, a solution of 1% Triton X- 100in PBS was applied for 1 h, followed by blocking in PBS containing 0.5% Triton X- 100 and fish skin gelatin (Electron Microscopy) for 1 h. Primary antibodies Chicken Anti- GFAP (Aves Lab., GFAP, 1:500), Rabbit Anti- IBA1 (FUJIFILM, 016- 20001, 1:200), Rabbit Anti- β- Catenin (Millipore, ABE208, 1:200), Rabbit Anti- NeuN (Cell Signaling Tech., D4G40, 1:200), |
