rabbit a myo7a  (Cell Signaling Technology Inc)

Journal: Frontiers in Cell and Developmental Biology

Article Title: Characterization and neurogenic responses of primary and immortalized Müller glia

doi: 10.3389/fcell.2025.1513163

Figure Lengend Snippet: Immunostaining results of neuron-like cells derived from primary MG cells with various neuronal markers and retinal neuron markers. (A) Cells were stained with TuJ1 marker and neuronal markers: NeuroD1 and Map2. Scale Bars, 50 μm. (B) Cells were stained with TuJ1 marker and retinal neuron markers: Calbindin (primarily expressed in horizontal cell, as well as in some subtypes of amacrine and retinal ganglion cells); Brn3a and Rbpms (markers of retinal ganglion cells); Otx2 (marker of bipolar and immature photoreceptor). Scale Bars, 50 μm. (C) Cells were stained retinal neuron markers: HuC/D (expressed in amacrine and retinal ganglion cells, with transient expression in horizontal cells during development), Rhodopsin (marker of mature rod photoreceptor). (D) The percentage of cells derived from primary MG that were positive for Calbindin/TuJ1 or HuC/D among total cells on day 7. The data are presented as mean ± SD (n = 3). Scale Bars, 50 μm. 6C–Medium supplemented with 6 small molecules.

Article Snippet: The primary antibodies used in this study were rabbit sex determining region Y (SRY)-box9 (Sox9; Merck, Cat. #AB5535, 1:100), rabbit glutamine synthetase (GS; Abcam, Cat. #ab73593, 1:100), rabbit glutamate aspartate transporter (GLAST; Frontier Institute, Cat. #GLAST-Rb-Af660, 1:100), rabbit glial fibrillary acidic protein (GFAP; CST, Cat. #12389, 1:100), mouse nestin (Invitrogen, Cat. #14-5,843-82, 1:500), rabbit paired box 6 (Pax6; BioLegend, Cat. #BL-901301, 1:300), mouse class III beta-tubulin (TuJ1; Genetex, GT11710, 1:500), rabbit RNA-binding protein with multiple splicing (Rbpms; Abcam, Cat. # Ab194213 , 1:200), rabbit Brn3a (Abcam, Cat. # Ab245230 , 1:500), rabbit Calbindin (Proteintech, Cat. # 14479-1-AP, 1:200), mouse Rhodopsin (Abcam, Cat. # ab3267, 1:200), rabbit microtubule-associated protein 2 (Map2; EMD Millipore, Cat. # AB5622, 1:200), mouse HuC/D (Santa Cruz Biotechnology, Cat. # sc-515624, 1:200), rabbit NeuroD1 (CST, Cat. #D90G12-7,019, 1:200), and rabbit Otx2 (PGI, Cat. #13497-1-AP, 1: 500).

Techniques: Immunostaining, Derivative Assay, Staining, Marker, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Characterization and neurogenic responses of primary and immortalized Müller glia

doi: 10.3389/fcell.2025.1513163

Figure Lengend Snippet: Immunostaining results of neuron-like cells derived from QMMuC-1 cells with various neuronal markers and retinal neuron markers. (A) Cells were stained with TuJ1 marker and neuronal markers: NeuroD1 and Map2. Scale Bars, 50 μm. (B) Cells were stained with TuJ1 marker and retinal neuron markers: Calbindin (primarily expressed in horizontal cell, as well as in some subtypes of amacrine and retinal ganglion cells); Brn3a and Rbpms (markers of retinal ganglion cells); Otx2 (marker of bipolar and immature photoreceptor). Scale Bars, 50 μm. (C) Cells were stained retinal neuron markers: HuC/D (expressed in amacrine and retinal ganglion cells, with transient expression in horizontal cells during development), Rhodopsin (marker of mature rod photoreceptor). Scale Bars, 50 μm. 6C–Medium supplemented with 6 small molecules.

Article Snippet: The primary antibodies used in this study were rabbit sex determining region Y (SRY)-box9 (Sox9; Merck, Cat. #AB5535, 1:100), rabbit glutamine synthetase (GS; Abcam, Cat. #ab73593, 1:100), rabbit glutamate aspartate transporter (GLAST; Frontier Institute, Cat. #GLAST-Rb-Af660, 1:100), rabbit glial fibrillary acidic protein (GFAP; CST, Cat. #12389, 1:100), mouse nestin (Invitrogen, Cat. #14-5,843-82, 1:500), rabbit paired box 6 (Pax6; BioLegend, Cat. #BL-901301, 1:300), mouse class III beta-tubulin (TuJ1; Genetex, GT11710, 1:500), rabbit RNA-binding protein with multiple splicing (Rbpms; Abcam, Cat. # Ab194213 , 1:200), rabbit Brn3a (Abcam, Cat. # Ab245230 , 1:500), rabbit Calbindin (Proteintech, Cat. # 14479-1-AP, 1:200), mouse Rhodopsin (Abcam, Cat. # ab3267, 1:200), rabbit microtubule-associated protein 2 (Map2; EMD Millipore, Cat. # AB5622, 1:200), mouse HuC/D (Santa Cruz Biotechnology, Cat. # sc-515624, 1:200), rabbit NeuroD1 (CST, Cat. #D90G12-7,019, 1:200), and rabbit Otx2 (PGI, Cat. #13497-1-AP, 1: 500).

Techniques: Immunostaining, Derivative Assay, Staining, Marker, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Characterization and neurogenic responses of primary and immortalized Müller glia

doi: 10.3389/fcell.2025.1513163

Figure Lengend Snippet: Immunostaining results of neuron-like cells derived from ImM10 cells with various neuronal markers and retinal neuron markers. (A) Cells were stained with TuJ1 marker and neuronal markers: NeuroD1 and Map2. Scale Bars, 50 μm. (B) Cells were stained with TuJ1 marker and retinal neuron markers: Calbindin (primarily expressed in horizontal cell, as well as in some subtypes of amacrine and retinal ganglion cells); Brn3a and Rbpms (markers of retinal ganglion cells); Otx2 (marker of bipolar and immature photoreceptor). Scale Bars, 50 μm. (C) Cells were stained retinal neuron markers: HuC/D (expressed in amacrine and retinal ganglion cells, with transient expression in horizontal cells during development), Rhodopsin (marker of mature rod photoreceptor). Scale Bars, 50 μm. 6C–Medium supplemented with 6 small molecules.

Article Snippet: The primary antibodies used in this study were rabbit sex determining region Y (SRY)-box9 (Sox9; Merck, Cat. #AB5535, 1:100), rabbit glutamine synthetase (GS; Abcam, Cat. #ab73593, 1:100), rabbit glutamate aspartate transporter (GLAST; Frontier Institute, Cat. #GLAST-Rb-Af660, 1:100), rabbit glial fibrillary acidic protein (GFAP; CST, Cat. #12389, 1:100), mouse nestin (Invitrogen, Cat. #14-5,843-82, 1:500), rabbit paired box 6 (Pax6; BioLegend, Cat. #BL-901301, 1:300), mouse class III beta-tubulin (TuJ1; Genetex, GT11710, 1:500), rabbit RNA-binding protein with multiple splicing (Rbpms; Abcam, Cat. # Ab194213 , 1:200), rabbit Brn3a (Abcam, Cat. # Ab245230 , 1:500), rabbit Calbindin (Proteintech, Cat. # 14479-1-AP, 1:200), mouse Rhodopsin (Abcam, Cat. # ab3267, 1:200), rabbit microtubule-associated protein 2 (Map2; EMD Millipore, Cat. # AB5622, 1:200), mouse HuC/D (Santa Cruz Biotechnology, Cat. # sc-515624, 1:200), rabbit NeuroD1 (CST, Cat. #D90G12-7,019, 1:200), and rabbit Otx2 (PGI, Cat. #13497-1-AP, 1: 500).

Techniques: Immunostaining, Derivative Assay, Staining, Marker, Expressing